Recombinant Rat Interleukin 6

• Cat.No.: Il6-98R
• Product Overview: Recombinant RatInterleukin-6 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21732 Dalton. The IL6 is purified by proprietary chromatographic techniques.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized without any additives.
• Solubility: It is recommended to reconstitute the lyophilized Interleukin-6 in 100 mM acetic acid to 1.0 mg/mL and incubated for 30 minutes at room temperature to regain full activity, which can then be further diluted to other aqueous solutions.
• Specific Activity: The ED₅₀= 0.03-0.1 ng/mL. The biological activity is determined by measuring the dose-dependant proliferation of IL-6 dapendent B9 cells. A concentration range of 0.1 to 10.0 ng/mL is effective for most in vitro applications.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard.
• Stability: Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: Il6 interleukin 6 [ Rattus norvegicus ]
• Synonyms: Il6; interleukin 6; ILg6; Ifnb2; Interleukin 6 (interferon, beta 2)
• Gene ID: 24498
• mRNA Refseq: NM_012589
• Protein Refseq: NP_036721
• UniProt ID: P20607
• Chromosome Location: 4q11
• Pathway: Cytokine-cytokine receptor interaction; Graft-versus-host disease; Hematopoietic cell lineage; Hypertrophic cardiomyopathy (HCM); Jak-STAT signaling pathway; Pathways in cancer; Prion diseases; Toll-like receptor signaling pathway
• Function: cytokine activity; growth factor activity; interleukin-6 receptor binding; protein binding; receptor binding