GMP Recombinant Human IL21

• Cat.No.: IL21-108H

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Interleukin-21 (IL-21) is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in na?ve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
• Form: Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
• Bio-activity: The ED50 determined by by a cell proliferation assay using human N1186 cells is less than 10 ng/mL, corresponding to a specific activity of >1.0 × 105 IU/mg.
• Molecular Mass: Approximately 15.4 kDa, a single non-glycosylated polypeptide chain containing 133 amino acids.
• AA Sequence: QGQDRHMIRM RQLIDIVDQL KNYVNDLVPE FLPAPEDVET NCEWSAFSCF QKAQLKSANT GNNERIINVS IKKLKRKPPS TNAGRRQKHR LTCPSCDSYE KKPPKEFLER FKSLLQKMIH QHLSSRTHGS EDS
• Endotoxin: Less than 1 EU/μg of IL-21 as determined by LAL method.
• Purity: >97% by SDS-PAGE and HPLC analyses.
• Usage: This material is offered by Creatvie Biomart for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.
• Storage Buffer: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at< -20°c.="" further="" dilutions="" should="" be="" made="" in="" appropriate="" buffered="">

Gene Information

• Gene Name: IL21 interleukin 21 [ Homo sapiens ]
• Official Symbol: IL21
• Synonyms: IL21; interleukin 21; interleukin-21; IL 21; Za11; interleukin-21 isoform; IL-21
• Gene ID: 59067
• mRNA Refseq: NM_001207006
• Protein Refseq: NP_001193935
• MIM: 605384
• UniProt ID: Q9HBE4
• Chromosome Location: 4q26-q27
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem
• Function: cytokine activity; cytokine receptor binding; interleukin-2 receptor binding