FabAffinity KBP Agarose High Flow

• Cat.No.: FKBP-002A
• Product Overview: Recombinant human Kappa Binding Protein is also named as KBP. FabAffinity KBP Agarose High Flow Resin, with KBP coupled to high flow Agarose matrix, is a new choice for purification of human antibodies and antibody fragments, which contain the constant domain of the Kappa light chain.

Specification

• Tag: Non
• Cat.no: FKBP-002A
• Form: Slurry in 20% Ethanol
• Size: 25 mL
• Matrix: Highly cross-linked Agarose
• Molecular Characterization: KBP is fused with a 6×his tag at C-terminus, and has a calculated molecular mass of 16 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of KBP is approximately 16 kDa.
• Average particle size: 90 μm
• Ligand: kappa binding protein (HEK derived)
• Coupling chemistry: Epoxy
• Dynamic binding capacity: 10mg Fab /ml media
• Recommended flow rate: 100-300cm/h
• Viscosity: Unused media can be stored in the container at a temperature of +4 to +8°C. Ensure that the screw top is fully tightened and keep container tightly closed in a dry and well-entilated place.Packed columns should be equilibrated in binding buffer containing 20% ethanol to prevent microbial growth.After storage, equilibrate with at least 5 bed volumes of starting buffer before use.
• Recommended column height: 5‐20cm
• pH working range: 3-10
• pH CIP range: 2-11
• CIP stability: 10mM NaOH, 6M Gua-HCl, 0.1M Acetate acid and 30% IPA
• Temperature Stability: 2‐40 °C
• Shelf life: 5 years