Recombinant Human EIF4E, His-tagged

• Cat.No.: EIF4E-2775H
• Product Overview: Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography. MW = 27.2 kDa(237aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-217 aa
• Description: EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
• Purity: > 85% by SDS - PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MATVEPETTP TPNPPTTEEE KTESNQEVAN PEH YIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVEDFWALYNHIQ LSSN LM PG CD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LET LL CLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Publications:
  Exonuclease resistant 18S and 25S ribosomal RNA components in yeast are possibly newly transcribed by RNA polymerase II (2020)

Gene Information

• Gene Name: EIF4E eukaryotic translation initiation factor 4E [ Homo sapiens ]
• Synonyms: eukaryotic translation initiation factor 4E; CBP; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; eukaryotic translation initiation factor 4E-like 1; eIF4E
• Gene ID: 1977
• mRNA Refseq: NM_001130678
• Protein Refseq: NP_001124150
• MIM: 133440
• UniProt ID: P06730
• Chromosome Location: 4q21-q25
• Pathway: Insulin signaling pathway; mTOR signaling pathway; Gene Expression; Metabolism of RNA; Metabolism of proteins; Signaling by Insulin receptor
• Function: translation initiation factor activity; protein binding; RNA cap binding

Q&As

Can the protein bind mRNA in vitro in the absence of ATP and other proteins?

EIF4E-2775H is a full-length protein that contains all binding sites; we highly recommend you try this protein.