Active Recombinant Zebrafish bmp2a, Met-tagged

• Cat.No.: bmp2a-366Z
• Product Overview: Recombinant Zebrafish bmp2a (Accession # AAI63048.1) Gln272-Arg386, fused with an N-terminal Met, was produced in E. coli-derived.

Specification

• Species: Zebrafish
• Source: E.coli
• ProteinLength: 272-386 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351.The ED50 for this effect is typically 100-400 ng/mL.
• Molecular Mass: Recombinant Zebrafish bmp2a, Met-tagged has a calculated MW of 13 kDa (monomer).
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: bmp2a bone morphogenetic protein 2a [ Danio rerio (zebrafish) ]
• Official Symbol: bmp2a
• Synonyms: bmp2a; bone morphogenetic protein 2a; bmp2; wu:fc59d09
• Gene ID: 30631
• mRNA Refseq: NM_131359
• Protein Refseq: NP_571434
• Chromosome Location: 17