Active Recombinant TEV Protease, His-tagged

• Cat.No.: Protease-23T
• Product Overview: Recombinant TEV protease contains an N-terminal His tag and can be easily removed by immobilized metal affinity chromatography (IMAC) from the cleavage reaction. The enzyme is compatible for both in-solution and on-column cleavage reactions. TEV Protease is purified from E. coli by proprietary chromatographic techniques and is >90% pure when visualized on an SDS-PAGE gel.

Specification

• Description: TEV Protease is a 27 kDa engineered form of Tobacco Etch Virus (TEV) protease. The optimum recognition site for TEV protease is the seven-amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ(G/S)] with cleavage occurring between the Gln and Gly/Ser residues. The enzyme is highly specific and active for its seven-amino acid sequence with minimal off-target effects which makes it an ideal choice to remove affinity tags from fusion proteins after protein purification. The optimal temperature for cleavage is 30 centigrade; however, it can be used at temperature as low as 4 centigrade.
• Source: E. coli
• Species: TEV
• Tag: His
• Bio-activity: 10,000 U/ml (~10,000 U/mg)
• Unit Definition: One unit of TEV Protease cleaves ≥85% of 3 μg control substrate in 1 hour at 30 centigrade.
  Unit Assay Conditions: The cleavage assay is performed in 1X TEV Buffer (50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA) and 1 mM DTT with 1 unit of enzyme and 3 μg control substrate at 30 centigrade for 1 hour in a total volume of 30 μl.
• Storage: Protease and the supplied components must be stored at -20 centigrade.
• Concentration: 10 Units/μL
• Storage Buffer: 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 1 mM DTT, and 50% Glycerol.
  TEV Protease is supplied with a vial of 20X TEV buffer (1 M Tris-HCl (pH 8.0), 10 mM EDTA) and a vial of 100 mM DTT.
  Highly specific cleavage activity
  Enhanced enzyme stability for prolonged protease activity
  Activity over a broad temperature (+4 centigrade to 30 centigrade) and pH (6.0 to 8.5) range
  His tag to facilitate its removal from the digested protein samples
• Shipping: Product requires shipping on ice packs.

Gene Information

• Official Symbol: Protease
• Synonyms: Protease