Active Recombinant Streptococcus pyogenes IdeS protein, His-tagged

• Cat.No.: IdeS-1013S
• Product Overview: Recombinant Streptococcus pyogenes IdeS protein(Asp 30 - Asn 339), fused with His tag, was expressed in E.coli .

Specification

• Species: Streptococcus pyogenes
• Source: E.coli
• Tag: His
• ProteinLength: 30-339 a.a.
• Form: Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.
• Bio-activity: BioactivityUnit Definition: One unit digests ≥ 95% of 1 μg human IgG when incubated in 10 mM sodium phosphate, 137 mM NaCl, 2.7 mM KCl, pH7.4 at 37°C for 30min.
• Molecular Mass: This protein carries a polyhistidine tag at the N-terminus.The protein has a calculated MW of 36.8 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE).
• Proteinlength: Asp30-Asn339
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.