Active Recombinant Rhesus EPHA4 Protein, Fc-tagged

• Cat.No.: EPHA4-1175R
• Product Overview: Recombinant rhesus EPHA4 (NP_001247799.1) (Met1-Thr547) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rhesus
• Tag: Fc
• Predicted N Terminal: Val 20
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized Rhesus EPHA4 at 10 μg/mL (100 μl/well) can bind biotinylated Rhesus EFNA5-Fc. The EC50 of biotinylated Rhesus EFNA5-Fc is 14.4-33.6 ng/mL.
• Molecular Mass: The recombinant rhesus EPHA4 comprises 769 amino acids and has a calculated molecular mass of 85.4KDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >96 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 1-547 a.a.

Gene Information

• Gene Name: EPHA4 EPH receptor A4 [ Macaca mulatta ]
• Official Symbol: EPHA4
• Synonyms: ephrin type-A receptor 4
• Gene ID: 704857
• mRNA Refseq: NM_001260870
• Protein Refseq: NP_001247799
• Chromosome Location: chromosome: 12
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem