Active Recombinant Renilla reniformis Luciferase (1-311), N-His-tagged

• Cat.No.: Luciferase-10R
• Product Overview: Recombinant renilla reniformis LUC (1-311), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Renilla reniformis
• Source: E.coli
• Tag: His
• Protein Length: 1-311
• Description: LUC, also known as luciferase. LUC catalyzes the oxidative decarboxylation of coelenterazine in the presence of dissolvedoxygen to yield oxyluciferin, CO2, and blue light. In vivo, the excited state luciferin-luciferase complex undergoes the process of noradiative energy transfer to an accessory protein, green fluorescent protein, which results in green bioluminescence. In vitro, it emits blue light in the absence of any green fluorescent protein.
• Form: Liquid
• Bio-activity: Specific activity is >1×10^9 lightunit/mg. One luciferase enzymeunit will produce one Relative Light Unit (RLU) at pH 7.5 at 25 centigrade.
• Molecular Mass: 38.5 kDa (335aa) Confirmed by MALDI-TOF
• AA Sequence: MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Gene Information

• Official Symbol: Luciferase
• Synonyms: Luciferase; Coelenterazine h 2-monooxygenase; EC:1.13.12.5; Renilla-luciferin 2-monooxygenase; Renilla-type luciferase
• UniProt ID: P27652