Recombinant Gaussia princeps Luciferase Protein, Avi-tagged, biotinylated

• Cat.No.: Luciferase-153
• Product Overview: Recombinant Gaussia princeps Luciferase fused with Avi tag((GLNDIFEAQKIEWHE) tag at C-terminal was expressed in E. coli. Biotinylated.

Specification

• Species: Gaussia princeps
• Source: E.coli
• Tag: Avi
• Description: Luciferase from Gaussia princeps was genetically engineered to be produced in E. coli and to contain the Avitag peptide sequence (GLNDIFEAQKIEWHE, C-terminus). The Avitag™ peptide was biotinylated so one biotin is covalently attached to the lysine within the Avitag peptide.
• Form: 50mM Tris/Cl pH 7.8, 600mM NaCl, 1mM EDTA, <10%BPER II (Pierce)
• Molecular Mass: 21,616 Da
• Purity: Gluc was purified via affinity
  chromatography to greater than 98% purity.
• Applications: Gaussia princeps luciferase provides all the research possibilities of the interaction between Streptavidin and biotin with the added applications of bioluminescence.
• Storage: The enzyme arrives on dry ice (or, in some cases, ice bricks) and should be immediately stored at -80 centigrade. Protein can be quickly thawed and placed at 4 centigrade. GLuc is stable at this temperature for at least 3 months. Avoid repeated freezethaw cycles.
• Shipping: Cold packs required. Store at -20 centigrade or below.