Active Recombinant Rat Neurofascin

• Cat.No.: Nfasc-14R
• Product Overview: Recombinant Rat Neurofascin, expressed in Mouse myeloma cell line, NS0 derived.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: His
• Description: Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins.1,2 The rat NF155 cDNA encodes a 1240 amino acid (aa) precursor that contains a 24 aa signal sequence, a 1086 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 109 aa cytoplasmic domain. The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif. A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain.3 Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155. NF155 is transiently expressed by oligodendr centigradeytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier.
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on 96 well culture plates that had been precoated with 50 μL/well of the rrNeurofascin solution at 1015 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 114.7 kDa.SDS PAGE: 135140 kDa, reducing conditions.
• Endotoxin: <1.0 eu per 1 μg of the protein by the lal</1.0
• Purity: >97%, by SDSPAGE under reducing conditions and visualized by silver stain.
• Usage: FOR RESEARCH USE ONLY
• Quality Control Test: 12 months from date of receipt, 20 to 70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, 20 to 70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Warning: Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Nfasc neurofascin [ Rattus norvegicus ]
• Official Symbol: Nfasc
• Synonyms: NFASC; neurofascin; NF
• Gene ID: 116690
• mRNA Refseq: NM_001160313
• Protein Refseq: NP_001153785
• UniProt ID: P97685
• Chromosome Location: 13q13
• Pathway: Axon guidance, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Developmental Biology, organism-specific biosystem; Interaction between L1 and Ankyrins, organism-specific biosystem; L1CAM interactions, organism-specific biosystem; Neurofascin interactions, organism-specific biosystem