Active Recombinant Rat ITGAV&ITGB6 protein, His-tagged

• Cat.No.: ITGAV&ITGB6-1595R
• Product Overview: Recombinant Rat ITGAV&ITGB6 protein(Phe31-Pro988(ITGAM)&Gly22-Pro708(ITGB6)), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 31-988;22-708 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human Fibronectin at 5 μg/mL (100 μL/well) can bind Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free with a linear range of 0.02-0.625 μg/mL.Immobilized Rat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag with a linear range of 0.3-10 ng/mL.
• Molecular Mass: Molecular CharacterizationRat ITGAV&ITGB6 Heterodimer, His Tag&Tag Free, has a calculated MW of 112.3 kDa (ITGAV) & 80.1 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus.
• Proteinlength: Phe31-Pro988(ITGAM)&Gly22-Pro708(ITGB6)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Itgav integrin, alpha V [ Rattus norvegicus ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin, alpha V; integrin alpha-V; Dnmt3l-ps1 pseudogene; Cd51
• Gene ID: 296456
• mRNA Refseq: NM_001106549
• Protein Refseq: NP_001100019