Active Recombinant Mouse ITGAV&ITGB6 protein, His-Avi-tagged, Biotinylated

• Cat.No.: ITGAV&ITGB6-1596M
• Product Overview: Biotinylated Recombinant Mouse ITGAV&ITGB6 protein(Phe31-Val988&Gly22-Pro708), fused with His and Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: 31-988;22-708 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Mouse Latent TGFB1, His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free with a linear range of 1-20 ng/mL.Immobilized Human Latent TGFB1, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free with a linear range of 1-20 ng/mL.
• Molecular Mass: Biotinylated Mouse ITGAV&ITGB6 Heterodimer Protein has a calculated MW of 114.2 kDa (ITGAV) & 80.2 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB6 contains no tag but a basic tail at the C-terminus.The protein migrates as 80-90 kDa and 135-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe31-Val988&Gly22-Pro708
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Itgav integrin alpha V [ Mus musculus ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin alpha V; integrin alpha-V; alphav-integrin; vitronectin receptor subunit alpha; vitronectin receptor alpha polypeptide (VNRA); CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• Gene ID: 16410
• mRNA Refseq: NM_008402
• Protein Refseq: NP_032428