Active Recombinant Rat ITGAV&ITGB5 protein, His-tagged

• Cat.No.: ITGAV&ITGB5-0280R
• Product Overview: Recombinant Rat ITGAV&ITGB5 protein(Phe31-Pro988(ITGAM)&Leu25-Asn719(ITGB5)), fused with His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• ProteinLength: 31-988;25-719 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Human Vitronectin, Tag Free at 5 μg/mL (100 μL/well) can bind Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free with a linear range of 0.02-0.156 μg/mL.Immobilized Biotinylated Human Vitronectin, Tag Free, primary amine labeling at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free with a linear range of 0.078-0.625 μg/mL.
• Molecular Mass: Rat ITGAV&ITGB5 Heterodimer, His Tag&Tag Free, has a calculated MW of 112.3 kDa (ITGAV) & 81.6 kDa (ITGB3). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB3 contains no tag but a basic tail at the C-terminus.
• Proteinlength: Phe31-Pro988(ITGAM)&Leu25-Asn719(ITGB5)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Itgav integrin, alpha V [ Rattus norvegicus ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin, alpha V; integrin alpha-V; Dnmt3l-ps1 pseudogene; Cd51
• Gene ID: 296456
• mRNA Refseq: NM_001106549
• Protein Refseq: NP_001100019