Active Recombinant Human ITGAV & ITGB5 Protein, His-Avi-tagged, Biotinylated

• Cat.No.: ITGAV & ITGB5-758H
• Product Overview: Biotinylated Human ITGAV & ITGB5 Heterodimer Protein is expressed from HEK293 cells. It contains AA Phe 31 - Val 992 (ITGAV) & Gly 24 - Asn 719 (ITGB5) (Accession # NP_002201.1 (ITGAV) & P18084-1 (ITGB5)). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag and subunit ITGB5 contains no tag but a basic tail at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 31-992;24-719 a.a.
• Predicted N Terminal: Phe 31 (ITGAV) & Gly 24 (ITGB5)
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5, 10% trehalose.
• Bio-activity: Immobilized Human Vitronectin at 5 ug/mL (100 uL/well) can bind Biotinylated Human ITGAV & ITGB5 Heterodimer Protein with a linear range of 0.01-0.156 ug/mL. Immobilized Human Fibronectin at 5 ug/mL (100 uL/well) can bind Biotinylated Human ITGAV & ITGB5 Heterodimer Protein with a linear range of 0.156-5 ug/mL.
• Molecular Mass: Biotinylated Human ITGAV & ITGB5 Heterodimer Protein, produced by co-expression of ITGAV and ITGB5, has a calculated MW of 114.7 kDa (ITGAV) and 81.9 kDa (ITGB5). The reducing protein migrates as 120 kDa and 150kDa (ITGAV) and 90 kDa (ITGB5) respectively due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Official Symbol: ITGAV; ITGB5
• Synonyms: Integrin alpha V beta 5, ITGAV & ITGB5