Active Recombinant Rat Il5 Protein (113 aa)
Cat.No. : | Il5-352I |
Product Overview : | Recombinant rat Interleukin-5 (rrIL-5) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 113 amino acids each. A fully biologically active molecule, rrIL-5 has a molecular mass of 26.0 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques. |
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Species : | Rat |
Source : | E.coli |
Protein Length : | 113 |
Description : | Interleukin-5 (IL-5), produced by mast cells, T cells and eosinophils, is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF). It can increase production and mobilization of eosinophils and CD34+ progenitors from the bone marrow. IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release. |
Form : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : | ED50 < 0.4 ng/mL, measured by a cell proliferation assay using TF-1 Cells, corresponding to a specific activity of > 2.5 × 10^6 units/mg. |
Molecular Mass : | 26.0 kDa, observed by non-reducing SDS-PAGE. |
AA Sequence : | MEIPMSTVVKETLIQLSTHRALLTSNETMRLPVPTHKNHQLCIGEIFQGLDILKNQTVRGGTVEILFQNLSLIKKYIDGQKEKCGEERRKTRHFLDYLQEFLGVMSTEWAMEV |
Endotoxin : | < 0.2 EU/μg, determined by LAL method. |
Purity : | > 95% by SDS-PAGE analysis. |
Storage : | Lyophilized recombinant rat Interleukin-5 (rrIL-5) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, rrIL-5 should be stable up to 2 weeks at 4 centigrade or up to 3 months at -20 centigrade. |
Storage Buffer : | Lyophilized after extensive dialysis against 20mM Tris, pH8.5. |
Reconstitution : | Reconstituted in ddH2O at 100 μg/mL. |
Gene Name | Il5 interleukin 5 [ Rattus norvegicus ] |
Official Symbol | Il5 |
Synonyms | IL5; interleukin 5; interleukin-5; TRF; IL-5; BCGF-II; B-cell growth factor II; T-cell replacing factor; cytotoxic T-lymphocyte inducer; eosinophil differentiation factor; Interleukin 5 (colony-stimulating factor eosinophil); Interleukin 5 (colony-stimulating factor, eosinophil); |
Gene ID | 24497 |
mRNA Refseq | NM_021834 |
Protein Refseq | NP_068606 |
UniProt ID | Q9R2C9 |
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Not For Human Consumption!
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