Active Recombinant Rat Il4r protein, hFc-tagged

• Cat.No.: Il4r-379R
• Product Overview: Recombinant Rat Il4r protein(Q63257-1)(Met1-Arg232), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Arg232
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized mouse IL4-His at 10 μg/mL (100 μl/well) can bind rat IL4R-Fc, The EC50 of rat IL4R-Fc is 0.12-0.29 μg/mL.
• Molecular Mass: The recombinant rat IL4R/Fc is a disulfide-linked homodimer. The reduced monomer comprises 448 amino acids and has a predicted molecular mass of 50.9 kDa. The apparent molecular mass of the protein is approximately 66 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il4r interleukin 4 receptor [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Il4r
• Synonyms: Il4r; Il4ra; interleukin 4 receptor; interleukin-4 receptor subunit alpha; IL-4RA; IL-4R-alpha; IL-4R subunit alpha; IL-4 receptor subunit alpha; interleukin 4 receptor, alpha; interleukin-4 receptor alpha chain
• Gene ID: 25084
• mRNA Refseq: NM_133380
• Protein Refseq: NP_596871
• UniProt ID: Q63257