Active Recombinant Rat ICOSLG protein, Fc-tagged

• Cat.No.: ICOSLG-2933R
• Product Overview: Recombinant Rat ICOSLG protein(Glu 25-Lys 261), fused with Fc tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Glu 25-Lys 261
• Form: Lyophilized from 0.22 μm filtered solution in 51 mM Tris,100 mM Glycine,25 mM Arginine,150 mM NaCl,pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Mouse ICOS (C137S, C138S), His Tag at 5 μg/mL (100 μL/well) can bind Rat B7-H2, Fc Tag with a linear range of 0.6-39 ng/mL.Immobilized Biotinylated Human ICOS (C136S, C137S), His,Avitag (recommended for biopanning) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Rat B7-H2, Fc Tag with a linear range of 2-39 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminusThe protein has a calculated MW of 53.4 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at-20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at:-20°C to-70°C for 12 months in lyophilized state;-70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Icoslg inducible T-cell co-stimulator ligand [ Rattus norvegicus ]
• Official Symbol: ICOSLG
• Synonyms: ICOSLG; inducible T-cell co-stimulator ligand; Icosl; RGD1562791
• Gene ID: 499415