Active Recombinant Human ICOSLG Protein, His-tagged

• Cat.No.: ICOSLG-1027H
• Product Overview: Recombinant Human ICOSLG (Accession # O75144) Asp19-Ser258, fused with a C-terminal 6-His tag, was produced in Human embryonic kidney cell, HEK293-derived.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Asp19-Ser258
• Predicted N Terminal: Asp19
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by its ability to co-stimulate IL-4 secretion by D10.G4.1 mouse helper T cells in the presence of anti-CD3.The ED50 for this effect is typically 0.3-1.5 µg/mL.Measured by its ability to stimulate human T cell proliferation in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808.The ED50 for this effect is typically 0.3-1.2 µg/mL.
• Molecular Mass: Recombinant Human ICOSLG, His-tagged has a calculated MW of 28 kDa. In SDS-PAGE migrates as 59-64 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ICOSLG inducible T-cell co-stimulator ligand [ Homo sapiens ]
• Official Symbol: ICOSLG
• Synonyms: ICOSLG; inducible T-cell co-stimulator ligand; ICOSL; ICOS ligand; B7 homolog 2; B7 homologue 2; B7 H2; B7 related protein 1; B7H2; B7RP 1; B7RP1; CD275; GL50; ICOS L; KIAA0653; B7-like protein Gl50; B7-related protein 1; transmembrane protein B7-H2 ICOS ligand; B7-H2; LICOS; B7RP-1; ICOS-L
• Gene ID: 23308
• mRNA Refseq: NM_015259
• Protein Refseq: NP_056074
• MIM: 605717
• UniProt ID: O75144
• Chromosome Location: 21q22.3