Active Recombinant Rat Efnb3 protein, hFc-tagged

• Cat.No.: Efnb3-8716R
• Product Overview: Recombinant Rat Efnb3 protein(NP_001094450)(Met1-Ser224), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Ser224
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized mouse EphB3-His (Cat:50581-M08H) at 10 μg/ml (100 μl/well) can bind rat EFNB3-Fc, The EC50 of rat EFNB3-Fc is 14.1-33.2 ng/ml.
• Molecular Mass: The recombinant rat EFNB3/Fc is a disulfide-linked homodimer. The reduced monomer comprises 438 amino acids and has a predicted molecular mass of 48.7 kDa. The apparent molecular mass of the protein is approximately 55 and 34 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Efnb3 ephrin B3 [ Rattus norvegicus ]
• Official Symbol: Efnb3
• Synonyms: EFNB3; ephrin B3; ephrin ligand B3; ELK-L3; RGD1565678
• Gene ID: 360546
• mRNA Refseq: NM_001100980
• Protein Refseq: NP_001094450