Active Recombinant Rat Cyp1b1 Protein
Cat.No. : | Cyp1b1-140R |
Product Overview : | Recombinant rat Cyp1b1 protein and CYP-reductase without tag were coexpressed in E. coli. |
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Species : | Rat |
Source : | E.coli |
Description : | Plays a role in polycyclic aromatic hydrocarbon metabolism in adrenal microsomes. |
Bio-activity : | Cytochrome c Reductase Activity: 1793 nmol/min/mg protein P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase]) Activity: 7-ethoxyresorufin O-deethylase Linear with time up to: 6 min(Determined at 0.1 µM substrate, using a P450 concentration of 1 pmol/ml) Linear with P450 up to: 10 pmol/ml(Determined at 0.1 µM substrate, using an incubation time of 1 min) Vmax: 2.1 pmol/min/pmol CYP Km: 0.09 µM |
Usage : | For laboratory (research) purposes only |
Storage : | Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice. |
Concentration : | P450 Concentration: 1.9 nmol/ml Protein Concentration: 11.6 mg/ml Specific P450 Content: 164 pmol/mg protein |
Storage Buffer : | 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA |
Gene Name | Cyp1b1 cytochrome P450, family 1, subfamily b, polypeptide 1 [ Rattus norvegicus (Norway rat) ] |
Official Symbol | Cyp1b1 |
Synonyms | Cyp1b1; cytochrome P450, family 1, subfamily b, polypeptide 1; cytochrome P450 1B1; CYPIB1; P450RAP; cytochrome P450, subfamily 1B, polypeptide 1; cytochrome P450RAP; hydroperoxy icosatetraenoate dehydratase |
Gene ID | 25426 |
mRNA Refseq | NM_012940 |
Protein Refseq | NP_037072 |
UniProt ID | Q64678 |
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Not For Human Consumption!
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