Active Recombinant Human CYP1B1 Protein

• Cat.No.: CYP1B1-01H
• Product Overview: Recombinant human CYP1B1 and human CYP-reductase without tag was coexpressed in Escherichia coli.
  Amount: 0.5 nmol cytochrome P450

Specification

• Species: Human
• Source: E.coli
• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.
• Bio-activity: Cytochrome c Reductase Activity: 44 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 50 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: 17β-estradiol 4-hydroxylase
  Linear with time up to: 30 min(Determined at 4 µM substrate, using a P450 concentration of 12.5 pmol/ml)
  Linear with P450 up to: 60 pmol/ml(Determined at 4 µM substrate, using an incubation time of 5 min)
  Vmax: 1.2 pmol/min/pmol CYP
  Km: 0.58 µM
• Usage: For laboratory (research) purposes only.
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 1.0 nmol/ml
  Protein Concentration: 10.0 mg/ml
  Specific P450 Content: 100 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP1B1 cytochrome P450 family 1 subfamily B member 1 [ Homo sapiens (human) ]
• Official Symbol: CYP1B1
• Synonyms: CYP1B1; cytochrome P450 family 1 subfamily B member 1; CP1B; ASGD6; GLC3A; CYPIB1; P4501B1; cytochrome P450 1B1; aryl hydrocarbon hydroxylase; cytochrome P450, family 1, subfamily B, polypeptide 1; cytochrome P450, subfamily I (dioxin-inducible), polypeptide 1 (glaucoma 3, primary infantile); dioxin-inducible cytochrome p450; flavoprotein-linked monooxygenase; hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; xenobiotic monooxygenase; EC 1.14.14.1; EC 4.2.1.152
• Gene ID: 1545
• mRNA Refseq: NM_000104
• Protein Refseq: NP_000095
• MIM: 601771
• UniProt ID: Q16678