Active Recombinant Rat Cd40lg, FLAG-tagged

• Cat.No.: Cd40lg-405R
• Product Overview: Rat CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG-tag.

Specification

• Species: Rat
• Source: CHO
• Tag: Flag
• Protein Length: 115-260;18-111 a.a.
• Description: MegaCD40L is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MegaCD40L has been shown to suppress alum-induced IL-1β release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.
• Form: Lyophilized. Contains PBS.
• Bio-activity: Induces B cell activation (as demonstrated by dose-dependent upregulation of CD86).
• Molecular Mass: ~35-40kDa
• Purity: ≥95% (SDS-PAGE)
• Stability: Stable for at least 6 months after receipt when stored at -20°C.
• Storage: Short Term Storage: +4°C; Long Term Storage: -20°C. After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions.
• Concentration: 0.1mg/ml after reconstitution.

Gene Information

• Gene Name: Cd40lg CD40 ligand [ Rattus norvegicus ]
• Official Symbol: Cd40lg
• Synonyms: CD40LG; CD40 ligand; CD40-L; CD40 ligand CD154; tumor necrosis factor ligand superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; Cd40l; Tnfsf5
• Gene ID: 84349
• mRNA Refseq: NM_053353
• Protein Refseq: NP_445805
• Pathway: Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem
• Function: CD40 receptor binding; CD40 receptor binding; CD40 receptor binding; cytokine activity; molecular_function; tumor necrosis factor receptor binding