Recombinant Rat Cd40lg Protein, Fc-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Cd40lg-545RAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Rat Cd40lg (NP_445805) (Gly115-Leu260), fused with the Fc region of human IgG1 at the N-terminus, was produced in Human Cells.

Specification

• Species: Rat
• Source: HEK293
• Tag: Fc
• Protein Length: 406
• Form: Lyophilized
• Molecular Mass: The recombinant rat CD40LG comprises 406 amino acids and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of the recombinant protein is approximately 53 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Purity: > 80 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from sterile PBS, pH7.4.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Cd40lg CD40 ligand [ Rattus norvegicus ]
• Official Symbol: Cd40lg
• Gene ID: 84349
• mRNA Refseq: NM_053353
• Protein Refseq: NP_445805