Active Recombinant Rat Btk protein, GST-tagged

• Cat.No.: Btk-185R
• Product Overview: Recombinant Rat Btk fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Rat
• Source: Insect Cells
• Tag: GST
• Description: BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation. BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors.Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. The unique role of BTK makes it a desirable target for potential anti-cancer, anti-inflammatory and anti-viral agents as well as other treatments.
• Form: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Bio-activity: 66 nmol/min/mg
• Molecular Mass: ~105 kDa
• Purity: >95%
• Applications: Kinase Assay, Western Blot
• Stability: 1yr at –70 centigrade from date of shipment
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 µg/µl

Gene Information

• Gene Name: Btk Bruton agammaglobulinemia tyrosine kinase [ Rattus norvegicus ]
• Official Symbol: Btk
• Synonyms: BTK; Bruton agammaglobulinemia tyrosine kinase; tyrosine-protein kinase BTK
• Gene ID: 367901
• mRNA Refseq: NM_001007798
• Protein Refseq: NP_001007799
• Chromosome Location: Xq34
• Pathway: B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem; Fc epsilon RI signaling pathway, conserved biosystem; IL-5 Signaling Pathway, organism-specific biosystem; IL-6 Signaling Pathway, organism-specific biosystem
• Function: identical protein binding; phosphatidylinositol-3,4,5-trisphosphate binding; protein tyrosine kinase activity; protein tyrosine kinase activity