Active Recombinant Full Length Human BTK Protein, DDK-tagged, Biotinylated

• Cat.No.: BTK-5615HF
• Product Overview: Full-length human BTK [2-659(end) amino acids and C481S of accession number NP_000052.1] was expressed as N-terminal DYKDDDDK tagged, biotinylated protein (80 kDa) using baculovirus expression system. The protein was purified by using DYKDDDDK tag antibody agarose.

Specification

• Species: Human
• Source: Sf21 Cells
• Tag: Flag
• ProteinLength: 2-659(end)
• Form: 50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
• Purity: 99%
• Applications: SDS-PAGE
• Storage: Store at -80C. Avoid repeating freeze-thaws.

Gene Information

• Gene Name: Bruton agammaglobulinemia tyrosine kinase
• Official Symbol: BTK
• Synonyms: BTK;AGMX1;AT;ATK;BPK;IGHD3;IMD1;PSCTK1;XLA
• Protein Refseq: NP_000052.1
• UniProt ID: Q06187