Active Recombinant Porcine IL1B2

• Cat.No.: IL1B2-90P
• Product Overview: Recombinant IL 1 beta Porcine produced in E.coli cells is a non-glycosylated, homodimeric protein containing 153 amino acid chain and having a molecular mass of 17.6kDa. The IL 1 beta is purified by proprietary chromatographic techniques.

Specification

• Species: Pig
• Source: E.coli
• Tag: Non
• ProteinLength: 153 a.a.
• Description: Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder. The IL 1 beta was lyophilized from a 0.2µm filtered concentrated solution in PBS pH 7.4, containing 3 % trehalose.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 10^5 IU/mg.
• Molecular Mass: 17.6kDa
• AA Sequence: ANVQSMECKL QDKDHKSLVL AGPHMLKALH LLTGDLKREV VFCMSFVQGD DSNNKIPVTL GIKGKNLYLS CVMKDNTPTL QLEDIDPKRY PKRDMEKRFV FYKTEIKNRV EFESALYPNW YISTSQAEQK PVFLGNSKGR QDITDFTMEV LSP
• Purity: Greater than 95.0% as determined by RP-HPLC and SDS-PAGE.
• Stability: Lyophilized IL 1 beta although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL 1 beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized IL 1 beta in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: IL1B2 interleukin 1, beta 2 [ Sus scrofa (pig) ]
• Official Symbol: IL1B2
• Synonyms: IL1B2; IL1B; IL-1beta; interleukin 1, beta 2; interleukin 1, beta; interleukin-1 beta
• Gene ID: 396565
• mRNA Refseq: NM_001005149
• Protein Refseq: NP_001005149
• Chromosome Location: 3
• Pathway: African trypanosomiasis; Chagas disease (American trypanosomiasis); Cytosolic DNA-sensing pathway