Active Recombinant Mouse Klk7 protein, His-tagged

• Cat.No.: Klk7-7405M
• Product Overview: Recombinant Mouse Klk7 protein(Q91VE3)(Met1-Arg249), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg249
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The specific activity is >70 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Molecular Mass: The recombinant mouse KLK7 comprises 239 amino acids and has a predicted molecular mass of 26.5 kDa. The apparent molecular mass of the protein is approximately 31 and 34 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Klk7 kallikrein related-peptidase 7 (chymotryptic, stratum corneum) [ Mus musculus ]
• Official Symbol: Klk7
• Synonyms: KLK7; kallikrein related-peptidase 7 (chymotryptic, stratum corneum); kallikrein-7; thymopsin; serine protease 6; protease, serine, 6; stratum corneum chymotryptic enzyme; kallikrein 7 (chymotryptic, stratum corneum); SCCE; Prss6
• Gene ID: 23993
• mRNA Refseq: NM_011872
• Protein Refseq: NP_036002