Active Recombinant Murine Lipocalin 2, GFP-tagged

• Cat.No.: Lcn2-2739M
• Product Overview: Recombinant murine NGAL (p25, SV-40-induced 24P3 protein, Lipocalin-2) is with a N-terminal GFP tag (Green fluorescent protein from Aequorea victoria). The expressed gene has been obtained merging the DNA codifying the GFP mutant at the N-term and the DNA of murine NGAL at C-term. The two proteins are connected by the linker GSAGSAAGAGAF. It is expressed inE. coli.

Specification

• Cat. No.: Lcn2-2739M
• Description: Lipocalin-2 also called Neutrophil Gelatinase Associated Lipocalin (NGAL) belongs to a family of lipocans which include 25 proteins (including α1-microglobulin and β-lactoglobulin), which are characterized by their ability to bind small lipophilic substances in their hydrophobic core. They thereby serve as transporters of substances like retinal, biliverdins & prostaglandins. There are indications that NGAL is involved in modulation of the inflammatory response and is found in the plasma of patients after stroke.
• Source: E. coli.
• Purity: > 95% by SDS-PAGE. Molecular weight > 45 kDa.
• Activity: Highly fluorescent GFP mutant (S65G, S72A) is a emits green light (emission maximum at 511 nm) when excited at 488 nm.
• Usage: NGAL GFP tagged Green Fluorescent Protein (GFP) is widely used as a fluorescent indicator for monitoring gene expression and protein ocalization in vivo.
• Storage: -20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20oC. Avoid repeated freeze/defrost cycles.

Gene Information

• Gene Name: Lcn2 lipocalin 2 [ Mus musculus ]
• Synonyms:
  Lcn2; lipocalin 2; 24p3; Sip24; AW212229; OTTMUSP00000013951; secreted inducible protein 24; NGAL
• Gene ID: 16819
• mRNA Refseq: 16819
• Protein Refseq: NP_032517
• UniProt ID: P11672
• Chromosome Location: 2 27.0 cM
• Function: binding; transporter activity