Active Recombinant Human Neutrophil gela-tinase-associated lipocalin

• Cat.No.: LCN2-4H
• Product Overview: The NGAL we can provide is humanized recombinant protein , and it has strong immunogenicity.

Specification

• Description: Neutrophil gelatinase-associated lipocalin (NGAL) is a 25KD glucoprotein and a new member of lipocalin family with a typical ?-barrel structure of the family. Based on the recent research, the function of NGAL protein may be is to protect and regulate the activity of matrix metalloproteinase-9(MMP-9) and to involve in inflammation and innate immunity reaction. NGAL protein also is a novel iron transporter that mediates a new iron delivery pathway.Acute kidney injury(AKI) is a syndrome characterized by loss of function in a short time ( hours to days ).The scarcity of early biomarker for AKI has hindered our ability to launch the therapy for this disease.Neutrophil gelatinase-assocliated lipocalin( NGAL ) is a high efficient clinical biomarker for AKI. At present , Cystatin C is widely used for AKI clinical diagnosis. However, compared to Cystatin C , NGAL diagnosis value for AKI is much higher .
• Source: E.coli
• Species: Human
• Predicted N Terminal: Gln 21
• Form: Liquid
• Bio-activity: Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2.
• Molecular Mass: The recombinant human NGAL consists of 197 amino acids. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhLCN2 is approximately 25 kDa as predicted.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95% as determined by SDS-PAGE
• Applications: It can be used to prepare high titer anti-NGAL antibody . And at the same time the NGAL protein can also be used as positive control in NGAL diagnosis Kit (renal function analysis , AKI analysis).
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 °C as supplied.3 months, -20 to -70 °C under sterile conditions after opening.
• Concentration: 1 mg/ml
• Storage Buffer: 20 mM Tris pH 7.5, 20% glycerol
• Tag: Non

Gene Information

• Gene Name: LCN2 lipocalin 2 [ Homo sapiens ]
• Official Symbol: LCN2
• Synonyms: LCN2; lipocalin 2; lipocalin 2 (oncogene 24p3); neutrophil gelatinase-associated lipocalin; 24p3; neutrophil gelatinase associated lipocalin; NGAL; oncogene 24p3; siderocalin; p25; lipocalin-2; migration-stimulating factor inhibitor; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; MSFI
• Gene ID: 3934
• mRNA Refseq: NM_005564
• Protein Refseq: NP_005555
• MIM: 600181
• UniProt ID: P80188
• Chromosome Location: 9q34
• Function: binding; iron ion binding; transporter activity