Active Recombinant Mouse Tnf Protein

• Cat.No.: Tnf-6540M
• Product Overview: Purified recombinant protein of Mouse tumor necrosis factor (Tnf) without tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: Leu80-Leu235
• Description: This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. Members of this family are classified based on primary sequence, function, and structure. This protein is synthesized as a type-II transmembrane protein and is reported to be cleaved into products that exert distinct biological functions. It plays an important role in the innate immune response as well as regulating homeostasis but is also implicated in diseases of chronic inflammation. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
• Bio-activity: The ED50 is 0.010-0.020 ng/mL, corresponding to a specific activity of 5-10 × 10^7 units/mg, determined by a dose dependent stimulation of L929 cells treated with actinomycin D.
• Molecular Mass: 17.3 kDa
• Endotoxin: Less than 0.01 ng/μg protein as determined by the LAL method
• Purity: > 98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 μg/mL in specified buffer
• Storage Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2

Gene Information

• Gene Name: Tnf tumor necrosis factor [ Mus musculus (house mouse) ]
• Official Symbol: Tnf
• Synonyms: TNF; tumor necrosis factor; TNF-a; TNF alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434
• Gene ID: 21926
• mRNA Refseq: NM_013693
• Protein Refseq: NP_038721
• UniProt ID: P06804