Recombinant Human Tumor Necrosis Factor

• Cat.No.: TNF-378H
• Product Overview: Recombinant human TNFalpha is expressed in human 293 cells and has been shown by gel filtration tobe predominantly a non-covalently linked homotrimer, with a molecular mass of51 kDa.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Tumor necrosisfactor-alpha is the best-known member of this class, and sometimes referredto when the term tumor necrosis factor is used. TNF is a monocyte-derivedcytotoxin that has been implicated in tumour regression, septic shock andcachexia.
• Purity: >95% The proteinwas resolved by SDS polyacrylamide gel electrophoresis and the gel wasstained with Coomassie blue.
• Molecular Mass: 17 kDa,non-disulfided bonded homotrimer, glycosylate.
• Endotoxin Level: Endotoxin Free <1EU/μg
• Biological Activity: 0.05 to 0.5 ng/mL ED50
• Specific Activity: The specificactivity was determined by the dose-dependent cytotoxity of the TNF alphasensitive cell line L-929 in the presence of Actinomycin D.
• OfficialSymbol: TNF
• Pathways: Adipocytokinesignaling pathway; Adipogenesis; African trypanosomiasis; Allograftrejection; Alzheimer" s disease; Amoebiasis; Amyotrophic lateral sclerosis(ALS); Angiopoietin receptor Tie2-mediated signaling; Antigen processing andpresentation; Apoptosis; Asthma; Calcineurin-regulated NFAT-dependenttranscription in lymphocytes; Canonical NF-kappaB pathway; Caspase cascade inapoptosis; Cellular roles of Anthrax toxin; Ceramide signaling pathway

Gene Information

• Gene Name: TNF tumor necrosis factor [ Homosapiens ]
• Synonyms: TNF;tumor necrosis factor; DIF; TNFA; TNFSF2; TNF-alpha; TNF-a; cachectin;APC1 protein; OTTHUMP00000029281; TNF, monocyte-derived; TNF,macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha;tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member2; Tumornecrosis factor; Cachectin
• Gene ID: 7124
• mRNA Refseq: NM_000594
• Protein Refseq: NP_000585
• MIM: 191160
• UniProt ID: P01375
• Chromosome Location: 6p21.3
• Function: cytokine activity; identicalprotein binding; protease binding; protein binding; transcription regulatoryregion DNA binding; tumor necrosis factor receptor binding