Active Recombinant Mouse Timp1 Protein, His-tagged

• Cat.No.: Timp1-6436M
• Product Overview: Purified recombinant protein of Mouse tissue inhibitor of metalloproteinase 1 (Timp1), transcript variant 2 with a C-His tag was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Cys27-Arg205
• Description: Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.
• Bio-activity: Mouse TIMP-1 inhibits the activity of activated mouse MMP-9 (100 ng/mL). The IC50 = 90 ng/mL.
• Molecular Mass: 22 kDa
• Endotoxin: Less than 1.0 EU/μg protein as determined by the LAL method
• Purity: > 90%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 μg/mL in specified buffer
• Storage Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0

Gene Information

• Gene Name: Timp1 tissue inhibitor of metalloproteinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Timp1
• Synonyms: TIMP1; tissue inhibitor of metalloproteinase 1; metalloproteinase inhibitor 1; EPA; TPA-S1; TPA-induced protein; erythroid-potentiating activity; collagenase inhibitor 16C8 fibroblast; tissue inhibitor of metalloproteinase-1; tissue inhibitor of metalloproteinases 1; Clgi; Timp; TIMP-1; MGC7143
• Gene ID: 21857
• mRNA Refseq: NM_011593
• Protein Refseq: NP_035723
• UniProt ID: P12032