Active Recombinant Mouse Timp1 protein

• Cat.No.: Timp1-3285M
• Product Overview: Recombinant Mouse Timp1 protein(NP_035723.2)(Met1-Arg205) was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Non
• Protein Length: Met1-Arg205
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2(R&D Systems, Catalog # ES001). The IC50 value is < 6 nM.
• Molecular Mass: The secreted recombinant mouse TIMP1 consists of 181 amino acids and has a calculated molecular mass of 21 kDa. It migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Timp1 tissue inhibitor of metalloproteinase 1 [ Mus musculus ]
• Official Symbol: Timp1
• Synonyms: TIMP1; tissue inhibitor of metalloproteinase 1; metalloproteinase inhibitor 1; EPA; TPA-S1; TPA-induced protein; erythroid-potentiating activity; collagenase inhibitor 16C8 fibroblast; tissue inhibitor of metalloproteinase-1; tissue inhibitor of metalloproteinases 1; Clgi; Timp; TIMP-1; MGC7143
• Gene ID: 21857
• mRNA Refseq: NM_001044384
• Protein Refseq: NP_001037849