Active Recombinant Mouse Sfrp5 Protein, HA-tagged

• Cat.No.: Sfrp5-712M
• Product Overview: Recombinant Mouse Sfrp5 (Accession # NP_061250) Ala22-His314, fused with an N-terminal HA (YPYDVPDYA) tag, was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Tag: HA
• Predicted N Terminal: Tyr (N-terminus of Hemagglutinin tag)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS and NaCl.
• Bio-activity: Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells.The ED50 for this effect is typically 0.1-0.5 µg/mL in the presence of 50 ng/mL Recombinant Mouse Wnt-3a.
• Molecular Mass: Recombinant Mouse Sfrp5, HA-tagged has a calculated MW of 34.3 kDa. In SDS-PAGE migrates as 39-42 kDa, reducing conditions.
• Protein length: Ala22-His314
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Sfrp5 secreted frizzled-related sequence protein 5 [ Mus musculus ]
• Official Symbol: Sfrp5
• Synonyms: SFRP5; secreted frizzled-related sequence protein 5; secreted frizzled-related protein 5; sFRP-5; SARP3; AI605071
• Gene ID: 54612
• mRNA Refseq: NM_018780
• Protein Refseq: NP_061250