Active Recombinant Mouse Sfrp2, His-tagged

• Cat.No.: Sfrp2-223M
• Product Overview: Recombinant Mouse Sfrp2 (Accession # AAB70795) Leu25-Cys295, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 25-295 a.a.
• Predicted N Terminal: Leu25
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured by its ability to compete with Frizzled-1 for binding to biotinylated Wnt-3a.The IC50 value is 0.7-3.5 nM, under conditions in which Recombinant Mouse (rm) Frizzled-1 Fc Chimera is present at 2.1 nM, and biotinylated rmWnt-3a concentration is 2.7 nM.
• Molecular Mass: Recombinant Mouse Sfrp2, His-tagged has a calculated MW of 31.6 kDa. In SDS-PAGE migrates as 35-40 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Sfrp2 secreted frizzled-related protein 2 [ Mus musculus ]
• Official Symbol: Sfrp2
• Synonyms: SFRP2; secreted frizzled-related protein 2; SARP-1; sFRP-2; stromal cell derived factor 5; secreted apoptosis-related protein 1; secreted frizzled-related sequence protein 2; secreted frizzled-related sequence protein 5; Sdf5; AI851596
• Gene ID: 20319
• mRNA Refseq: NM_009144
• Protein Refseq: NP_033170