Active Recombinant Mouse Sell Protein, His & Fc-tagged

• Cat.No.: Sell-831M
• Product Overview: Recombinant extracellular domain (Met 1-Asn 332) of mouse SELL (NP_035476.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His&Fc
• Predicted N Terminal: Trp 39
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to SELL coated plates (10 μg/mL, 100 μL/well) approximately >60% cells will adhere specifically.
• Molecular Mass: The recombinant mouse SELL/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 542 amino acids and has a predicted molecular mass of 61 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm SELL/Fc monomer is approximately 100-110 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Protein length: 1-332 a.a.

Gene Information

• Gene Name: Sell selectin, lymphocyte [ Mus musculus ]
• Official Symbol: Sell
• Synonyms: SELL; selectin, lymphocyte; L-selectin; LAM-1; LECAM1; lymphocyte antigen 22; lymph node homing receptor; leukocyte adhesion molecule 1; CD62 antigen-like family member L; lymphocyte surface MEL-14 antigen; leukocyte-endothelial cell adhesion molecule 1
• Gene ID: 20343
• mRNA Refseq: NM_001164059
• Protein Refseq: NP_001157531
• Pathway: Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis,
• Function: binding; cell adhesion molecule binding; glycolipid binding; protease binding; protein binding; sugar binding