Active Recombinant Mouse MMP9 protein, His-tagged

• Cat.No.: MMP9-1312M
• Product Overview: Recombinant Mouse MMP9 protein(Ala 20 - Pro 471), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Ala 20 - Pro 471
• Form: Supplied as 0.2 μm filtered solution in 25 mM Tris, 150 mM NaCl, pH7.5, 50% Glycerol with trehalose as protectant.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >1,500 pmol/min/μg.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 52.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Mmp9 matrix metallopeptidase 9 [ Mus musculus ]
• Official Symbol: MMP9
• Synonyms: MMP9; matrix metallopeptidase 9; matrix metalloproteinase-9; GELB; Gel B; gelatinase B; 92kD gelatinase; 92kDa gelatinase; 92 kDa gelatinase; collagenase type IVB; 92kD type IV collagenase; 92kDa type IV collagenase; 92 kDa type IV collagenase; matrix metalloproteinase 9; Clg4b; MMP-9; B/MMP9; AW743869; pro-MMP-9
• Gene ID: 17395
• mRNA Refseq: NM_013599
• Protein Refseq: NP_038627