Active Recombinant Mouse LTA4H protein, His-tagged

• Cat.No.: LTA4H-859M
• Product Overview: Recombinant Mouse LTA4H protein(NP_032543.2)(Met1-Asp611), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (R-AMC). The specific activity is >15 pmoles/min/μg.
• Molecular Mass: The recombinant mouse LTA4H consists of 621 amino acids and has a calculated molecular mass of 70.4 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Met1-Asp611
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Lta4h leukotriene A4 hydrolase [ Mus musculus ]
• Official Symbol: LTA4H
• Synonyms: LTA4H; leukotriene A4 hydrolase; leukotriene A-4 hydrolase; LTA4 hydrodase; LTA-4 hydrolase
• Gene ID: 16993
• mRNA Refseq: NM_008517
• Protein Refseq: NP_032543