Active Recombinant Human LTA4H protein, His-tagged

• Cat.No.: LTA4H-305H
• Product Overview: Recombinant Human LTA4H protein(NP_000886.1)(Met1-Asp611), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin(R-AMC). The specific activity is > 15 pmoles/min/μg.
• Molecular Mass: The recombinant human LTA4H consists of 621 amino acids and predicts a molecular mass of 70.7 kDa. It migrates as approximately 58-68 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Met1-Asp611
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LTA4H leukotriene A4 hydrolase [ Homo sapiens ]
• Official Symbol: LTA4H
• Synonyms: LTA4H; leukotriene A4 hydrolase; leukotriene A-4 hydrolase; LTA-4 hydrolase; FLJ17564
• Gene ID: 4048
• mRNA Refseq: NM_000895
• Protein Refseq: NP_000886
• MIM: 151570
• UniProt ID: P09960