Active Recombinant Mouse Kitl Protein

• Cat.No.: Kitl-3698M
• Product Overview: Purified recombinant protein of Mouse kit ligand (Kitl / SCF ) without tag was expressed in E. coli.

Specification

• Description: Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
• Source: E. coli
• Species: Mouse
• Bio-activity: The ED50 is 15 ng/mL, corresponding to a specific activity of 6.7 × 10^4 units/mg, determined by the dose dependent stimulation of TF-1 cell proliferation.
• Molecular Mass: 18.3 kDa
• Protein length: Lys26-Ala189
• Endotoxin: Less than 0.01 ng/μg protein as determined by the LAL method
• Purity: > 98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 μg/mL in specified buffer
• Storage Buffer: 10 mM NaHPO4 pH 7.2, 0.15 M NaCl

Gene Information

• Gene Name: Kitl kit ligand [ Mus musculus (house mouse) ]
• Official Symbol: Kitl
• Synonyms: KITL; kit ligand; cloud gray; C-kit ligand; Steel factor; grizzle-belly; stem cell factor; mast cell growth factor; hematopoietic growth factor KL; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; Kitlg; contrasted
• Gene ID: 17311
• mRNA Refseq: NM_013598
• Protein Refseq: NP_038626
• UniProt ID: P20826