Active Recombinant Human KITLG protein, His-tagged

Cat.No. : KITLG-2655H
Product Overview : Active Recombinant Human c-Kit ligand/SCF/KITLG Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Glu26-His214) of human SCF/C-kit ligand (Accession #NP_000890.1) fused with a 6×His tag at the C-terminus.
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Species : Human
Source : HEK293
Tag : His
Protein Length : Glu26-His214
Form : Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Bio-activity : 1.Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.08-0.3 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized recombinant human SCF at 1 μg/mL (100 μL/well) can bind recombinant human CD117 with a linear range of 1-6 ng/mL.
Molecular Mass : 30-40 kDa
Endotoxin : < 0.1 EU/μg of the protein by LAL method.
Purity : > 97% by SDS-PAGE.
Storage : Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
Reconstitution : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Gene Name KITLG KIT ligand [ Homo sapiens ]
Official Symbol KITLG
Synonyms KITLG; KIT ligand; MGF; kit ligand; familial progressive hyperpigmentation 2; FPH2; Kitl; KL 1; mast cell growth factor; SCF; SF; steel factor; stem cell factor; c-Kit ligand; KL-1; SHEP7; kit-ligand; DKFZp686F2250;
Gene ID 4254
mRNA Refseq NM_000899
Protein Refseq NP_000890
MIM 184745
UniProt ID P21583

Not For Human Consumption!

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