Active Recombinant Mouse Il7 Protein (Glu26-Ile154), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il7-62M
• Product Overview: Recombinant Mouse Il7 Protein (Glu26-Ile154) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Glu26-Ile154
• Description: Interleukin-7 (IL-7) is a multipotent cytokine belonged to one of the members of IL-2 superfamily. IL-7 has diverse effects on the hematopoietic and immune regulations. IL-7 is a trophic factor that is necessary for both B cell and T cell proliferation and development. In addition, it presents potential antitumor effects in tumors such as glioma, melanoma, lymphoma, leukemia, prostate cancer, and glioblastoma.
• Form: Lyophilized
• Bio-activity: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBMC). The ED50 for this effect is < 0.2 ng/mL. The specific activity of recombinant mouse IL-7 is > 5 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 15.8 kDa.
  The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il7 interleukin 7 [ Mus musculus (house mouse) ]
• Official Symbol: Il7
• Synonyms: IL7; interleukin 7; interleukin-7; Il-7; hlb368; A630026I06Rik; MGC129342
• Gene ID: 16196
• mRNA Refseq: NM_008371
• Protein Refseq: NP_032397
• UniProt ID: P10168