Active Recombinant Rat Il6 Protein

• Cat.No.: Il6-176I
• Product Overview: Recombinant Rat Il6 Protein without tag was expressed in HEK 293.

Specification

• Species: Rat
• Source: HEK293
• Description: Interleukin-6 (IL-6) is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, Interleukin-6 (IL-6) has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. Interleukin-6 (IL-6) signals through the IL-6 receptor system that consists of two chains, IL-6R alpha and gp130.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 3 pg/mL, measured by a cell proliferation assay using 7TD1 Cells.
• Molecular Mass: 21~26 kDa, observed by reducing SDS-PAGE.
• AA Sequence: FPTSQVRRGDFTEDTTHNRPVYTTSQVGGLITYVLREILEMRKELCNGNSDCMNSDDALSENNLKLPEIQRNDGCFQTGYNQEICLLKICSGLLEFRFYLEFVKNNLQDNKKDKARVIQSNTETLVHIFKQEIKDSYKIVLPTPTSNALLMEKLESQKEWLRTKTIQLILKALEEFLKVTMRSTRQT
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant Rat Interleukin-6(IL-6) remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, it should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: Il6 interleukin 6 [ Rattus norvegicus ]
• Official Symbol: Il6
• Synonyms: IL6; interleukin 6; interleukin-6; IL-6; Interleukin 6 (interferon, beta 2); ILg6; Ifnb2
• Gene ID: 24498
• mRNA Refseq: NM_012589
• Protein Refseq: NP_036721
• UniProt ID: P20607