Active Recombinant Mouse Il6 Protein

• Cat.No.: Il6-114M
• Product Overview: Purified recombinant protein of Mouse interleukin 6 (Il6) (Phe25-Thr211) without tag was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 25-211 a.a.
• Description: This gene encodes a member of the interleukin family of cytokines that have important functions in immune response, hematopoiesis, inflammation and the acute phase response. The ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration and wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Bio-activity: The ED50 is less than 0.01 ng/ml, corresponding to a specific activity of > 1 x 10^8 units/mg, determined by the dose dependent stimulation of 7TD1 cell proliferation.
• Molecular Mass: 21.9 kDa
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Storage Buffer: Sodium Acetate and EDTA

Gene Information

• Gene Name: Il6 interleukin 6 [ Mus musculus (house mouse) ]
• Official Symbol: Il6
• Synonyms: Il6; interleukin 6; Il-6; interleukin-6; B-cell hybridoma growth factor; interleukin HP-1
• Gene ID: 16193
• mRNA Refseq: NM_031168
• Protein Refseq: NP_112445
• UniProt ID: P08505