Active Recombinant Mouse Il28b Protein (Asp20-Val193), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il28b-153M
• Product Overview: Recombinant Mouse Il28b Protein (Asp20-Val193) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin 28B (IL-28B) predicts a molecular mass of 19.7 kDa. IL-28 comes in two isoforms, IL-28A and IL-28B, and plays a role in immune defense against viruses, including the induction of an "antiviral state" by turning on Mx proteins, 2',5'-oligoadenylate synthetase as well as ISGF3G.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measure by its ability to protect HepG2 cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is < 20 ng/mL.
• Molecular Mass: The protein has a calculated MW of 20.56 kDa.
  The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis).
• Protein length: Asp20-Val193
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 2 weeks under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il28b interleukin 28B [ Mus musculus (house mouse) ]
• Official Symbol: Il28b
• Synonyms: IL28B; interleukin 28B; interleukin-28B; IFN-lambda-3; interleukin 28; interferon alpha; interferon lambda-3; Il28; IFL-1; IL-28B; INF-alpha; INF-lambda
• Gene ID: 338374
• mRNA Refseq: NM_177396
• Protein Refseq: NP_796370
• UniProt ID: Q8CGK6