Active Recombinant Mouse Il12a&Ebi3, HIgG1 Fc-tagged

• Cat.No.: Il12a&Ebi3-111M
• Product Overview: A soluble dimeric fusion protein consisting of the extracellular domain of Mouse IL12a subunit (NP_032377.1)(Arg23-Ala215) was fused to human IgG1 Fc, and the mouse Ebi3 subunit (NP_056581.1)(Ala23-Pro228) linked to IL12a by disulfide bonds.

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• Protein Length: 23-215;23-228 a.a.
• Form: Lyophilized from 0.2μm-filtered solution in PBS.
• Bio-activity: Bioactivity was measured in a cell proliferation assay of Con A activated mouse splenocytes.
• Molecular Mass: 46kDa and 23kDa (monomer)
• AA Sequence: A soluble dimeric fusion protein consisting of the extracellular domain of Mouse IL12a subunit (NP_032377.1)(Arg23-Ala215) was fused to human IgG1 Fc, and the mouse Ebi3 subunit (NP_056581.1)(Ala23-Pro228) linked to IL12a by disulfide bonds.
• Endotoxin: <0.06 eu/μg="" as="" determined="" by="" lal="">
• Purity: >98%, by SDS-PAGE under reducing conditions.
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Reconstitution: Reconstitute at 100μg/ml in sterile PBS.
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: Il12a&Ebi3
• Official Symbol: IL35
• Synonyms: p35; Ll12a; Il-12a; IL-12p35; EBI-3; IL-27
• Gene ID: 16159 & 50498
• mRNA Refseq: NM_001159424&NM_015766
• Protein Refseq: NP_001152896& NP_056581