Active Recombinant Human EBI3&IL12A, MIgG2a Fc-tagged

• Cat.No.: EBI3&IL12A-121H
• Product Overview: The human IL-35 complex composed of the Ebi3 subunit(NP_005746.2) (Arg21-Lys229) and the IL-12a(p35) subunit (NP_000873.2)(Arg57-Ser253) is fused through a polypeptide linker to the Fc portion of mouse IgG2awas expressed in CHO cell

Specification

• Species: Human
• Source: CHO
• Tag: Fc
• ProteinLength: 21-229;57-253 a.a.
• Description: The gene EBI3 was identified by its induced expression in B lymphocytes in response Epstein-Barr virus infection. It encodes a secreted glycoprotein belonging to the hematopoietin receptor family, and heterodimerizes with a 28 kDa protein to form interleukin 27 (IL-27). IL-27 regulates T cell and inflammatory responses, in part by activating the Jak/STAT pathway of CD4+ T cells, while the other gene IL12A encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.
• Form: Lyophilized from 0.2μm-filtered solution in PBS.
• Bio-activity: Bioactivity was measured in a cell proliferation assay using anti-CD3 activated human peripheral mononuclear cells.
• Molecular Mass: 75 KDa (monomer)
• AA Sequence: The human IL-35 complex composed of the Ebi3 subunit(NP_005746.2) (Arg21-Lys229) and the IL-12a(p35) subunit (NP_000873.2)(Arg57-Ser253) is fused through a polypeptide linker to the Fc portion of mouse IgG2a.
• Endotoxin: <0.06 eu/μg="" as="" determined="" by="" lal="" test.="">
• Purity: >98%, by SDS-PAGE under reducing conditions.
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Reconstitution: Reconstitute at 100μg/ml in sterile PBS.
• Warning: Avoid freeze/thaw cycles.

Gene Information

• Gene Name: EBI3; IL12A
• Official Symbol: IL35
• Synonyms: P35; CLMF; NFSK; NKSF1; IL-12A;IL27B; IL-27B; EBI3; Epstein-Barr virus induced 3
• Gene ID: 10148; 3592
• mRNA Refseq: NM_000882& NM_005755
• Protein Refseq: NP_005746& NP_000873
• MIM: 161560& 605816
• UniProt ID: Q14213&P29459
• Chromosome Location: 3q25.33&19p13.3