Active Recombinant Mouse Il10 Protein (Ser19-Ser178), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il10-45M
• Product Overview: Recombinant Mouse Il10 Protein (Ser19-Ser178) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Ser19-Ser178
• Description: Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. Many different types of cells can produce IL-10, including immune cells and non-immune cells. IL-10 exerts inhibitory functions on DCs and macrophages, which is a potent inhibitor of antigen presentation and limit the production of the Th1-associated cytokines IL-2 and interferon-γ (IFN-γ). IL-10 is also a key immunoregulator during infection due to its inhibitory effect on inflammatory cytokine production. Consequently, the excessive Th1 and CD8+ T cell responses could be suppressed during infection.
• Form: Lyophilized
• Bio-activity: Measure by its ability to induce MC/9‑2 cells proliferation. The ED50 for this effect is < 1 ng/mL. The specific activity of recombinant mouse IL-10 is > 1 × 10⁶ IU/mg.
• Molecular Mass: The protein has a calculated MW of 19.7 kDa.
  The protein migrates as 19 kDa under reducing condition (SDS-PAGE analysis).
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il10 interleukin 10 [ Mus musculus (house mouse) ]
• Official Symbol: Il10
• Synonyms: IL10; interleukin 10; interleukin-10; cytokine synthesis inhibitory factor; CSIF; Il-10
• Gene ID: 16153
• mRNA Refseq: NM_010548
• Protein Refseq: NP_034678
• UniProt ID: P18893