Source :	HEK293
Species :	Mouse
Tag :	Non
Form :	Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity :	1. Measured by its binding ability in a functional ELISA. Immobilized Mouse Interferon Gamma at 2 μg/ml (100 μl/well) can bind Mouse IFNGR1 hFc, the EC50 of Mouse IFNGR1 hFc is7.0-30.0 ng/mL.2. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV).The ED50 for this effect is 0.05-0.3 ng/mL.
Molecular Mass :	The recombinant mouse IFNG consists of 133 amino acids and predicts a molecular mass of 15.5 kDa. As a result of different glycosylation, it migrates as approximately 19.6 and 15.4 kDa band in SDS-PAGE under reducing conditions.
Protein length :	Met1-Cys155
Endotoxin :	< 1.0 EU per μg of the protein as determined by the LAL method
Purity :	> 85 % as determined by SDS-PAGE
Storage :	Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Name	Ifng interferon gamma [ Mus musculus ]
Official Symbol	Ifng
Synonyms	IFNG; interferon gamma; IFN-gamma; gamma interferon; Ifg; IFN-g;
Gene ID	15978
mRNA Refseq	NM_008337
Protein Refseq	NP_032363